Isolation and Identification of Diatom from Urban lake water
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Published
Sep 20, 2021
Manjunatha Reddy
Smita Kavadikeri
Sumathra Manokaran
Abstract
Diatoms are primary producers of freshwater. Many diatom species are classified based on their morphological characteristics only, because of their distinctive structure of frustules. Yet this approach requires core expectance taxonomic. The use of molecular analysis techniques in combination with morphological identification provides good aid to the taxonomical recognition of diatoms. The fresh water diatom were collected from urban lakes of Bangalore, Karnataka, India. Using Pasture pipette method, the Unialgal Culture was obtained. For fresh water diatom Chu#10medium with pH 7 incubated at 20°C was used. Morphological diversity of diatom species was observed under microscopy. DNA extracted and purified then subjected to 28S rRNA sequencing with primer. PhyML is used for phylogenetic relationships of these strains to 28S rRNA gene sequences. The results of 28S rRNA sequence base pairs with adjacent sequence of the species determined under the order of Bacillariales and found that 99% Nitzschia palea and 98% Navicula cryptocephala.
How to Cite
Reddy, M., Kavadikeri, S., & Manokaran, S. (2021). Isolation and Identification of Diatom from Urban lake water. SPAST Abstracts, 1(01). Retrieved from https://spast.org/techrep/article/view/1037
Article Details
Keywords
Diatoms, rRNA, phylogenetic tree, taxonomy, sequence
References
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9. Edlund, M. B. & Spaulding, S. A. Initial observations on uniparental auxosporulation in Muelleria (Frenguelli in Muelleria (Frenguelli) Frenguelli and Scoliopleura Grunow (Bacillariophyceae); pp.213-225 (2006).
10. Fawley, M., Fawley, K., & Buchheim, M. (2004). Molecular diversity among communities of freshwater micro chlorophytes. Microbial Ecology, 48(4), 489-499 (2004).
11. Florence C. H. Lee and Vickneshwaran Muthu, From 18S to 28S rRNA Gene: An Improved Targeted Sarcocystidae PCR Amplification, Species Identification with Long DNA Sequences, Am. J. Trop. Med. Hyg., 104(4), (2021).
12. Irena Kaczmarska, Charlotte Reid, Monica Moniz -Diatom taxonomy: morphology, molecules and barcodes- Proceedings of the 1st Central European Diatom Meeting, (2007).
13. Ishii K-I, Iwataki M, Matsuoka K, Imai I. Proposal of identification criteria for resting spores of Chaetoceros species (Bacillariophyceae) from a temperate coastal sea. Phycologia; 50(4): 351–62, (2011).
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16. Guillard RRL. Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH, editors. Culture of Marine Invertebrate Animals. New York: Plenum Press; pp. 29–60(1975).
17. Gina V. Filloramo, Bruce A. Curtis, Emma Blanche & John M. Archibald, Re-examination of two diatom reference genomes using long-read sequencing, BMC Genomics, 379 (2021).
18. Eric J. Carpenter, Naim Matasci, Saravanaraj Ayyampalayam, Shuangxiu Wu, Jing Sun, Jun Yu, Fabio Rocha Jimenez Vieira, Chris Bowler, Richard G. Dorrell, Matthew A. Gitzendanner, Ling Li, Wensi Du, Kristian K. Ullrich, Norman J. Wickett, Todd J. Barkmann,Michael S. Barker, James H. Leebens-Mack and Gane Ka-Shu Wong Access to RNA-sequencing data from 1,173 plant species: The 1000 Plant transcriptomes initiative (1KP), GigaScience, 8, (2019).
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21. Maria Valeria Ruggiero, Domenico D'Alelio, Maria Immacolata Ferrante, Mariano Santoro, Laura Vitale, Gabriele Procaccini and Marina Montreso. Clonal expansion behind a marine diatom bloom- The ISME Journal 12: 463–472 (2018).
22. Stein, J (ED.) Handbook of Phycological methods. Culture methods and growth measurements. Cambridge University Press. 448, (1973).
23. Sámed I. I. A. Hadi, Hugo Santana, Patrícia P. M. Brunale, Taísa G. Gomes, Márcia D. Oliveira, Alexandre Matthiensen, Marcos E. C. Oliveira, Flávia C. P. Silva, Bruno S. A. F. Brasil. PLOS ONE.
Section
ES: Environmental Sciences