Anticancer and Antimicrobial studies of Stannane of D Biotin

Abstract

This research work was intended at developing a novel drug that could act as an anticancer and antimicrobial agent. A well-established method was used to synthesize stannane of D Biotin, in a benzene-ethanol medium, by azeotropic removal of the water molecules in the reaction system.

The refluxing was carried out initially with Dean-Stark attachment followed by the use of Rotatory Vacuum Evaporator for the removal of any solvent left in the system and its characterization was carried using spectral analysis (1H NMR and FTIR studies). Invitro antibacterial study of the complex is carried against, Bacillus subtilis MTCC121       (gram-positive) and Pseudomonas aeruginosa 1934 (gram-negative) bacterial strains using the ‘well diffusion method’. The experiential results were further compared with the control (chloramphenicol) after 24 hours of incubation. The synthesized complex inhibited the bacterial growth of Bacillus subtilis MTCC121      (gram-positive) and Pseudomonas aeruginosa 1934         (gram-negative) in vitro antibacterial study.   In silico docking studies, were carried out using computational software iGemDock v2.1 tool, and its interaction with PDB 1 m14 (Tyrosine Kinase Domain from Epidermal Growth Factor Receptor) was studied. In insilico study, the amino acid of the tyrosine kinase domain of EGFR showed effective binding with synthesized complex and it inhibited the overexpression of epidermal growth factor receptor (EGFR) which results in various cancers like breast, lung, ovary, and prostate. The result gave a suggestion that the complex could be used to prevent dimerization of protein and hence it has a chemotherapeutic efficacy on Tyrosine Kinase Domain from Epidermal Growth Factor Receptor. Thus the novel complex was found to be a good anticancer agent.