Development of lanthanum oxide nanoparticles-based biosensor for highly selective and sensitive detection of ciprofloxacin in milk

Main Article Content

Article Sidebar

Published Sep 8, 2021
NAVNEET CHAUDHARY AMIT K. YADAV
JAI GOPAL SHARMA PARTIMA R. SOLANKI

Abstract

Antibiotics are being used widely in veterinary practices for the therapeutic management of livestock and the inhibition of microbial infections. Over-use and possibly fraudulent misuse of these antibiotics as dietary growth promoters necessitate the development of surveillance and monitoring technologies [1]. The over-usage of drugs has led to antibiotic resistance in bacteria, creating challenges for many societies, hospitals, and health centers due to an increase in overall patient numbers and costly treatment [2]. Ciprofloxacin (CPX) [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl) 3-quinolone carboxylic acid], is a very stable antibiotic that does not go through complete metabolism in the body, and 30-90 % of CPX remains constantly [3]. The growing public concern of accumulation of drug residues in the food supply chain and livestock has led to the general administration of this antibiotic [4-6]. There is a strong probability that it reaches the environment through patients’ urine samples and wastewater due to inadequate metabolization of CPX in the body, which induces antibiotic resistance. In the coming future, the phenomenon of antibiotic resistance will be more complex.[7] As a result, there is an urgent requirement to closely track the use and discharge of these medications in the atmosphere via the human body. Several techniques for the detection of CPX have been used so far, including spectrophotometry [8], liquid chromatography-mass spectrophotometry [9], immunoassay, chemiluminescence, capillary electrophoresis [10], and electrochemical techniques [11]. The disadvantage of these approaches is that they are all time-consuming, costly, complex methods, and they all necessitate advanced automation and the assistance of skilled users. To resolve this problem, various efforts have been made to develop simple, rapid, inexpensive, and sensitive bio/sensors for CPX detection electrochemically. In this study, nanostructured lanthanum oxide nanoparticles (nLa2O3 NPs) were synthesized by the co-precipitation method, which is considered the facile and quickest nanoparticles synthesis process [12]. As-synthesized nLa2O3 NPs have been characterized with various analytical and spectroscopic and morphological techniques such as X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, contact angle, transmission electron microscopy, and electrochemical methods. These nLa2O3 NPs were used for enhancing the conductivity of the immunosensor. In brief, nLa2O3 NPs were functionalized by APTES and were deposited electrophoretically on the surface of ITO glass substrate followed by immobilizing anti-CPX antibodies covalently via EDC-NHS chemistry. Blocking of the non-specific area was done with the help of BSA. Further, the nLa2O3 NPs have significant physio-electrochemical properties that have resulted in developing a highly efficient electrochemical biosensor for the detection of CPX having excellent sensitivity, repeatability, stability, and selectivity. The change in electrochemical response studies of the developed immunosensor (BSA/anti-CPX/APTES/nLa2O3/ITO) was monitored by differential pulse voltammetry (DPV) to detect CPX. Under optimized conditions, studies showed that the developed biosensor had a wide linear detection range of 0.001-0.5 ng mL−1 and 1-1000 ng mL−1, a lower detection limit of 1 pM mL-1 with two sensitivities of 11.44 mA ng-1 mL cm-2 (R2 of 0.968), and 7.88 mA ng-1 mL cm-2 (R2=0.972). The developed immunosensor showed good reproducibility, repeatability, sensitivity, and stability that was effectively explored to detect CPX in milk samples. To the best of our knowledge, this is the first work on the development of an electrochemical-based immunosensor for the detection of CPX using nLa2O3 NPs.

How to Cite

CHAUDHARY, N., YADAV, A. K., SHARMA, J. G., & SOLANKI, P. R. (2021). Development of lanthanum oxide nanoparticles-based biosensor for highly selective and sensitive detection of ciprofloxacin in milk. SPAST Abstracts, 1(01). Retrieved from https://spast.org/techrep/article/view/183
Abstract 12 |

Article Details

References
[1] C.A. Michael, D. Dominey-Howes, M. Labbate, The antimicrobial resistance crisis: causes, consequences, and management, Frontiers in public health 2 (2014) 145.
[2] W.H. Organization, Antimicrobial resistance and primary health care, World Health Organization, 2018.
[3] J. Syska, Frieden wave-function representations via an Einstein-Podolsky-Rosen-Bohm experiment, Physical Review E 88(3) (2013) 032130.
[4] W. Hall, Superbugs: An arms race against bacteria, Harvard University Press2018.
[5] P.K. Mutiyar, A.K. Mittal, Risk assessment of antibiotic residues in different water matrices in India: key issues and challenges, Environmental Science and Pollution Research 21(12) (2014) 7723-7736.
[6] J. Fick, H. Söderström, R.H. Lindberg, C. Phan, M. Tysklind, D.J. Larsson, Contamination of surface, ground, and drinking water from pharmaceutical production, Environmental Toxicology and Chemistry 28(12) (2009) 2522-2527
[7] N. Kemper, Veterinary antibiotics in the aquatic and terrestrial environment, Ecological indicators 8(1) (2008) 1-13.
[8] T. Wang, H. Yin, Y. Zhang, L. Wang, Y. Du, Y. Zhuge, S. Ai, Electrochemical aptasensor for ampicillin detection based on the protective effect of aptamer-antibiotic conjugate towards DpnII and Exo III digestion, Talanta 197 (2019) 42-48.
[9] R. Yuan, Z. Yan, A. Shaga, H. He, Design and fabrication of an electrochemical sensing platform based on a porous organic polymer for ultrasensitive ampicillin detection, Sensors and Actuators B: Chemical 327 (2021) 128949
[10] S.J. Clarke, R.E. Littleford, W.E. Smith, R. Goodacre, Rapid monitoring of antibiotics using Raman and surface enhanced Raman spectroscopy, Analyst 130(7) (2005) 1019-1026.
[11] M.M. Abdelrahman, I.A. Naguib, M.A. Elsayed, H.A. Zaazaa, Chromatographic methods for quantitative determination of ampicillin, dicloxacillin and their impurity 6-aminopenicillanic acid, Journal of chromatographic science 56(3) (2018) 209-215.
[12] S. Solé, A. Merkoci, S. Alegret, New materials for electrochemical sensing III. Beads, TrAC Trends in Analytical Chemistry 20(2) (2001) 102-110.
Section
SE1: Sensors