In-silico structural modelling and prediction of conformational B- cell epitopes of potential vaccine candidate PRE- binding protein

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Published Aug 25, 2021
BIJARA SANASAM Sanjeev Kumar

Abstract

Malaria is a life threatening infectious disease causes by Plasmodium species, wherein Plasmodium falciparum being the most lethal causes severe malaria [1,2]. Plasmodium parasites have a complex life cycle and employed several immunity evading mechanism that enable the parasite to multiply and survive avoiding the host immunity [3]. Alternative pathway and multiple proteins are available to achieve similar function if any pathway got blocked which makes vaccine development a difficult task [4]. The rise in drug-resistant Plasmodium parasites and the unavailability of an effective vaccine lead to major challenges in controlling the parasite. Drug resistance has been also reported towards Artemisinin combination therapies (ACTs), which is the most accepted and recommended therapy, giving a major impact to the human health [5]. And RTS,S/AS01, which is the only accepted vaccine, has low efficacy and doesn’t provide full protection [6]. Therefore, it is of utmost important to identify novel potential targets that can be directed for therapeutic intervention [7]. The present study hypothesized highly conserved protein, PRE-binding protein (PREBP), as a potential vaccine candidate for malaria vaccine development. PREBP is an exposed surface protein with high antigenic property and conservancy among other species of the parasite. In the present study, conservancy, localisation and the antigenicity of PREBP was determined by using different bioinformatics tools. And the 3D structure of PREBP (Figure1) was modelled using Robetta tool [8] and the quality of the generated model was further validated by generating Ramachandran plot . Conformational B cell epitopes of the protein were predicted using Ellipro [9]. The generated 3-D structure of the protein can be further used for other structural analysis and docking studies. And the resulted epitopes might be of great importance as a vaccine subunit in the development of an effective malaria vaccine.

How to Cite

SANASAM, B., & Kumar, S. (2021). In-silico structural modelling and prediction of conformational B- cell epitopes of potential vaccine candidate PRE- binding protein. SPAST Abstracts, 1(01). Retrieved from https://spast.org/techrep/article/view/122
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Keywords

PREBP, vaccine, epitopes, Plasmodium falciparum, malaria

References
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Section
NB:Biology